Catalog Number: RK42017-010: For 50-100 IHC Tests with frozen or FFPE tissues
Package Size: RK42017-005: For 25-50 IHC Tests with frozen or FFPE tissues
Concentration: Ready to use
Storage: Store at 4°C upon receiving. DO NOT FREEZE.
Intended Use: For Research Use Only. This product is designed and developed to meet the need for ultra-fast immunohistochemistry (IHC) testing at convenient room temperature. The assay is optimized to work on frozen tissue sections using a 10 minute IHC protocol.
Introduction: The key component in this kit is horseradish peroxidase (HRP) polymer (PolyHRP) labeled Rabbit anti-human AKT1 monoclonal antibody clone R252. The product has been developed using Novodiax’s proprietary enzyme polymerization technology and Direct IHC technology called ihcDirect (Patent Pending) and is optimized for frozen tissues. The incubation time on frozen tissues is 3 minutes and on FFPE tissues 15-30 minutes. AKT1 is a serine/threonine-protein kinase and was originally identified as an oncogene in a transforming retrovirus. Depending upon different post-translational modifications, AKT1 can be detected in cytoplasm, nucleus and cell membrane in all human cell types so far analyzed. Mutations and site-specific phosphorylation of AKT1 have been reported to associate with high-level expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. Its defects have been reported to associate with colorectal cancer and ovarian cancer.
ihcDirect® AKT1 (Rabbit monoclonal antibody Clone R252)
- Catalog Number: RK42017
- Availability: In Stock
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