Catalog Number: E28001-C500
Specificity: Mouse antibody IgG
Suggested Dilutions: ELISA: 5,000x-15,000x
Storage: 4°C upon receiving. Do not freeze.
It is well known that the second antibody conjugated with polymerized HRP provides much higher detection sensitivity than the antibody conjugated with monomer HRP. In comparison with biotinylated antibody/HRP-streptavidin two-step detection system, the polyHRP-antibody one step detection system simplifies ELISA assay and saves assay times without compromising detection sensitivity.This product is developed based on Novodiax’s proprietary Supersensitive Polymer HRP Detection Platform and optimized for ELISA. Highly polymerized HRPs were conjugated to goat anti mouse IgG second antibodies. It offers ultra-high sensitivity for detecting mouse IgG primary antibody. Through the development process, some leading commercial products on current market have been chosen to compare with. Our results show Novodiax’s polyHRP-second antibody conjugates are the best in this class.
Mouse IgG was coated on ELISA plate in different concentrations. PolyHRP-secondary antibody conjugates from different suppliers were diluted at the same factor, and then added into the plate.