For Research Use Only. Not for use in diagnostic procedures.
Novodiax pHRP goat anti-rabbit IgG is a polymerized horseradish peroxidase (HRP) labeled secondary antibody that provides ultra-high detection sensitivity while maintaining low background
Background:
It is very-well known that pHRP labeled secondary antibody product provides higher detection sensitivity than regular HRP-antibody conjugates. It also usually provides the same or superior result than the biotin/streptavidin two-step detection system, with reduced assay step and without suffering from the background caused by endogenous biotin, thus the assay is simplified and accelerated in comparison to the conventional procedure with biotin/streptavidin detection system.
This product is developed based on Novodiax’s proprietary and sensitive polymer HRP detection platform and optimized for IHC. Through the development process, the leading commercial products in market have been chosen as quality standards to compete with, which ensured pHRP products the best in class.
Features:
High sensitivity: ~30X amplifications by pHRP to boost signals in detecting low-abundant targets.
Low background: Amplifications without background from endogenous biotin.
Fast: Eliminates one incubation step.
Versatile: compatible with IHC, ELISA, and Western blotting.
Application Details:
IHC for multiple biomarkers: Antigen Retrieval with pH 6.0 Citrate buffer + T20 for 30 min in a 96?C water bath, block with 1% BSA, incubation with primary antibody for 30 min at room temperature, incubation with pHRP Goat Anti-Rabbit for 30 min at room temperature; DAB staining and hematoxylin counterstaining.
